IUBio

Seeking: E.coli strain allowing inverted repeated DNA

S. Findley polliwog at u.washington.edu
Mon Mar 15 05:28:09 EST 1999


I am looking for a cloning strain of E.coli which will 
permit the growth of two identical but inverted copies of the same
fragment i.e.:
<<<<<<<<<<<<<<<X>>>>>>>>>>>>>>>>>

I vaguely recall a series of vectors designed a few years ago with huge
inverted polylinkers that could grow deletion-free in a particular strain
of bug...


Please email me if you have a lead on such a strain

-Thanks

SDF
-- 
Seth D. Findley               polliwog at u.washington.edu
Department of Biochemistry    Phone: 206-543-1710
Health Sciences Building J579
University of Washington, Box 357350, Seattle, WA 98195



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