In article <7cjsgn$lq4$1 at nntp4.u.washington.edu>,
polliwog at u.washington.edu (S. Findley) wrote:
>> I am trying to amplify fragments of 2-3 kB with NEB's Vent polymerase.
> I have heard that this enzyme doesn't like to please researchers trying to
> amplify longer fragments.
>> What conditions have proven successful in optimizing/extending Vent
Mixing with Taq polymerase, for one!
The long PCR protocols typically use between 1% and 10% Taq polymerase in
conjunction with Vent (NEB) or Pwo (Boehringer) or Pfu (Stratagene). This
way you get the error correction of the high-fidelity enzym and the proces-
sivity of Taq.
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