Calculation of Primer Tm value

Andrew Leung leungkc at glink.net.hk
Mon Mar 15 06:27:54 EST 1999

Hi all,

    I know that there are many program which is designed for estimating the
Tm value of specific primer sequence. However, different programs may yeild
different Tm value based on there particular default parameters. If my
primers are used for standard PCR, what parameters should I use? Is there
anyone out there having comment on this.


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