IUBio

Request: Vent PCR: advice on optimizing Longish PCR

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Tue Mar 16 07:06:07 EST 1999


In article <7ck8d8$s22$1 at nnrp1.dejanews.com>, ladasky at my-dejanews.com
writes
>
>The long PCR protocols typically use between 1% and 10% Taq polymerase in
>conjunction with Vent (NEB) or Pwo (Boehringer) or Pfu (Stratagene).  This
>way you get the error correction of the high-fidelity enzym and the proces-
>sivity of Taq.

Wrong way around. 1-10% proof-reading to 90-99% Taq.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk



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