PIPES solution (How do you get the PIPES to disolve?)

ÃÖ ¿õ khlee at MED.CHUNGBUK.AC.KR
Tue Mar 16 05:35:47 EST 1999


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<HTML>
If you want to use this PIPES buffer for E. coli transformation buffer,
you should use KOH instead of NaOH to adjust pH (please refer to the book
of Sambrook.)
<BR>Just add almost equimolar amount of KOH and you can get clear solution
with pH less then 6.8. After that&nbsp; you can finely adjust pH to 6.8.

<P>Woong Choi</HTML>

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