protein gel mixtures

Hiranya S. Roychowdhury hroychow at NMSU.EDU
Tue Mar 16 12:11:58 EST 1999


At 10:47 AM 3/16/99 +0100, J.S. Good wrote:
>Any reason why one shouldn't mix acrylamide, buffer, water, SDS and use
>this as as a stock solution, adding APS and TEMED at the last minute to an
>aliquot prior to casting the gel?
>
>

Reasons for not doing this:
1. You may not use the same percentage of gel every time.
2. You need to store acrylamide in the cold and SDS comes out of solution in
cold. You don't want to warm the acrylamide solution repeatedly.
3. You really do not need much time to mix the stock solutions up for making
the gel. If you must, you may make up the "working buffer" solutions (ie.
Tris.Cl and SDS) for both the resolving and the stacking gels at 4x
concentration and store those indefinitely (making sure there is no change
in conc. through evap.). Indeed, this is a common practice in many labs that
run a lot of gels each week.


Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu




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