G418, Yeast genetic background difference? Others?

Hui Cai hcai at ucla.edu
Tue Mar 16 11:47:30 EST 1999

Hello All.
  I had a couple of quick questions, please, if anybody could help out.

1)  Is there a genetic background effect of G418 resistance using the
NeoR gene?
(I have a diploid strain with the resistance that grows fine on
YPD+G418, but,
after I sporulate none of the spores will grow on YPD+G418)

2)  Possibly just a historical question, but, why does it seem that
antibiotic resistance
markers are used so seldomly in yeast work?

Thanks for your help!
    hcai at ucla.edu

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