protein expression in PLysS

kandasamy ravi ravi77 at MAILCITY.COM
Tue Mar 16 17:21:44 EST 1999


Sir,

   I have cloned my gene in pET15b transformed in to DH5 and isolated DNA and
retransformed in to pLysS BL21(DE3).checked the sequences(it is in right frame)
But i don't get overexpression just little increase in expression.and the uninduced 
one is expressing as good as induced.I tried different IPTG concentration but no
use and even the story is same in C43/C41 cell lines.
    what do i do to get over expression?and why there is a leaky expression in 
uninduced control.
somebody help me please...
All suggestion welcome

Thank you
K.Ravi


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