IUBio

Extra sequence in PCR

David Diamond rddiamond at earthlink.net
Tue Mar 16 14:48:01 EST 1999


Has anyone seen or can they explain the following:

In amplifying a segment of a gene using primers based on a related
species,  cloning the amplified product, and sequencing it I have found
apparently authentic sequence (15-20 bases) on the 5' side of the primers.

Thanks

David Diamond
rddiamond at earthlink.net




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