Tissue preservation for DNA

Ross S Feldberg rfeldber at emerald.tufts.edu
Tue Mar 16 08:24:35 EST 1999


I am interested in preserving tissue samples from wild amphibians for
later DNA extraction.  Any suggestions for an effective preservation
solution?

In particular, I have already mixed a solution of 20% DMSO, 0.25M EDTA,
and NACL sat.  I was advised that this might be more effective than 95%
ETHANOL.  Is there any reason not to use this solution?  Will it work
equally well to preserve nuclear and mitochodrial DNA?

Jon Regosin
Dept. of Biology
Tufts University
jregos01 at tufts.edu




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