RNA injection into the mouse oocytes

Sandra Wong syywong at hkusua.hku.hk
Wed Mar 17 03:26:27 EST 1999


Dear user,

I am going to do the microinjection of RNA into the mouse oocytes.  Does
anyone has a protocol that I can follow? 
My plasmid is T7-IRES-Cre. I need a protocol for RNA synthesis for
injection. If you have the protocol. Kindly please send this to me.
I also have few questions to ask.  The RNA that I synthesis is dissolve in
what kind of solution for injection.  Do I need to cap the RNA for the
stability?

Best regards,
Sandra Wong
email address: syywong at hkusua.hku.hk



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