double signal in chromos. FISH ?

Carsten Hohoff hohoff at uni-muenster.de
Wed Mar 17 10:01:10 EST 1999


Dear experts in the field of chromosomal FISHing !

I expected a double-signal on metaphase chromosomes (due to the occurrence
of the gene on both chromatides) - but what I observe in nearly half of the
chromosomes inspected is that there is just ONE fluorescence signal !

What imay be the reason ? Is the chromatide DNA not denatured well enough ?

Any helpful comment is appreciated 

Carsten Hohoff




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