"Autogen" Miniprep Method (?)
dneece at uiuc.edu
Wed Mar 17 16:26:36 EST 1999
Does anyone know the procedure for doing minipreps similar to the
"Autogen" method? Basically, the Autogen does the miniprep in a total of
two tubes, and is not able (as far as I know) to pipet off the aqueous
layer in a phenol extraction -- it somehow combines the phenol with the
KOAc step. What I'm looking for is a way to perform Alk Lys (including
phenol ext.) with a minimal number of tube changes and hassles. I found a
list of Autogen reagents on their internet site and pasted them below. I
believe they are listed in the order added.
70% Alcohol Wash
Anyone have any ideas?
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