high background in northerns

William Sun william at neuro.usc.edu
Wed Mar 17 15:52:56 EST 1999

Hello all,

Most lab manuals suggest that with a high specific activty probe (>10^9dpm/ug)
one should not add more than 10^6 dpm/ml of probe to a northern blot.  Too
high a probe concentration would lead to high background.  What is the 
theoretical basis for this?  If a membrane is properly blocked, why would 
high probe concentration still lead to high background?  

William Sun, Ph.D               
Metabasis Therapeutics, Inc.           Phone: (619) 622-3930
9390 Towne Centre Drive                FAX:   (619) 622-5545
San Diego, CA 92121                    Email: william at alum.mit.edu 

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net