IUBio

Request: Vent PCR: advice on optimizing Longish PCR

ladasky at my-dejanews.com ladasky at my-dejanews.com
Tue Mar 16 18:13:21 EST 1999


In article <ZEbuQGAvkk72Ew7o at genesys.demon.co.uk>,
  "Dr. Duncan Clark" <Duncan at genesys.demon.co.uk> wrote:
> In article <7ck8d8$s22$1 at nnrp1.dejanews.com>, ladasky at my-dejanews.com
> writes
> >
> >The long PCR protocols typically use between 1% and 10% Taq polymerase in
> >conjunction with Vent (NEB) or Pwo (Boehringer) or Pfu (Stratagene).  This
> >way you get the error correction of the high-fidelity enzym and the proces-
> >sivity of Taq.
>
> Wrong way around. 1-10% proof-reading to 90-99% Taq.
>
> Duncan
> --
> The problem with being on the cutting edge is that you occasionally get
> sliced from time to time....

     Whoops!  Of course you're right.  And today, I got sliced!


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