In article <ZEbuQGAvkk72Ew7o at genesys.demon.co.uk>,
"Dr. Duncan Clark" <Duncan at genesys.demon.co.uk> wrote:
> In article <7ck8d8$s22$1 at nnrp1.dejanews.com>, ladasky at my-dejanews.com> writes
> >The long PCR protocols typically use between 1% and 10% Taq polymerase in
> >conjunction with Vent (NEB) or Pwo (Boehringer) or Pfu (Stratagene). This
> >way you get the error correction of the high-fidelity enzym and the proces-
> >sivity of Taq.
>> Wrong way around. 1-10% proof-reading to 90-99% Taq.
> The problem with being on the cutting edge is that you occasionally get
> sliced from time to time....
Whoops! Of course you're right. And today, I got sliced!
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