M9 plate problem

Jesse J. Parry casshan at siu.edu
Thu Mar 18 00:10:42 EST 1999


Long <longli at itsa.ucsf.edu> wrote in message
news:36E566B4.C4EFCA0E at itsa.ucsf.edu...
>Hi, All
>
>I got a very basic question about M9 plate. Several months ago, I made a
>batch of M9 plates which worked very well. This time, I made another
>batch just following the same receipe and I got a kind of dark brown
>liquid after autoclaving. Bacteria refused to grow on them. I remembe
>the first time I prepared M9 and got a almost clear liquid. So I figured
>I must have added something wrong and repeated once. the result was the
>same. Could someone tell me what color should be the final M9 plate?
>
>Thanks
>
>Long
>UCSF
>
>
You have to autoclave M9 by itself and then add it to autoclaved agar.  You
should make 40X M9 and then add 12.5 ml to 500 ml agar.  You get the point.
M9 should be clear, but the agar plates will be milky colored (depending on
the Carbon source used).

Jesse Parry
casshan at siu.edu






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