Expression COXIII mRNA Questions

Alan J Watson watsonaj at bms.com
Thu Mar 18 08:49:11 EST 1999

Awhile ago we probed a Northern with an EST that was actually a
chimera of the clone we wanted and COXIII.  It now behooves us to
analyze this blot in greater detail.  I really don't want to
repeat the experiment the right way (it's not *that* important!),
so my questions are:

Can I use the signal from the COXIII as a loading control, or is
expression of COXIII tissue-dependent in some way?

Also,we picked up two bands from the COXIII portion of the probe
- does anyone know what the "correct" size of the COXIII message

Thanks in advance,


A. John Watson, Ph.D.
Bristol-Myers Squibb Co.
Neuroscience Drug Discovery
Wallingford, CT 06492

watsonaj at bms.com

"If you're not part of the solution, you're part of the

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net