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Transformation Efficiency

Dr Antony Halsall halsalla at cf.ac.uk
Thu Mar 18 15:26:05 EST 1999


Hi all,

I am transforming CaCl competent cells and would be grateful if anyone could tell me, or 
point me in the right direction on, how to calculate the transformation efficiency of my 
cells. I am/may be foced to blunt end ligate and therefore want cells with a high 
transformation efficiency. Our copy of Current Protocols in Molecular Biology is missing 
the vital pages.

Thanks for any help

Dr Antony Halsall
Dept Psychological Medicine
UWCM



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