Primer3 Exclusion Parameters

Dr. Paul N. Hengen pnh at GALLO.UCSF.EDU
Thu Mar 18 19:48:05 EST 1999

Does anyone know how to force the Primer3 program
to exclude certain repeat regions in a PCR product?
I'm using the Boulder IO format to run large batches
of DNA fragments through Primer3, but I want to exclude
human repeat elements such as Alu/Lines and I want to
exclude extensive polyA, polyT regions from the expected
PCR product. If you have experience with this, please
send me E-mail. Thanks!


Paul N. Hengen, Ph.D.
Department of Neurology
Gallo Clinic and Research Center
San Francisco, CA 94110 USA
phone: (415) 648-7111 x458
fax:   (415) 648-7116
mailto:pnh at

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