IUBio

RT-PCR of GC rich regions

amherrera at CIGB.EDU.CU amherrera at CIGB.EDU.CU
Fri Mar 19 09:06:38 EST 1999


    Hi all, 
    I need to amplify GC rich regions (%GC = 80) by RT-PCR and I 
    have run with troubles using the conventional procedures. 
    
    It seem to me that someone once recomended the use of 
    some aditives like formamide but I do not remeber well.
    
    Could anybody lend me a working protocol to solve this? 
    
    Many thanks to all
    DiogenesQV



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