IUBio

cDNA Spun Columns, SizeSep 400 Spun Columns, etc.

lyubo1 at my-dejanews.com lyubo1 at my-dejanews.com
Fri Mar 19 12:30:06 EST 1999


Hello,

Have someone used Pharmacia's cDNA Spun Columns (with Sephacryl S-300) and/or
SizeSep 400 Spun Columns (with Sepharose CL-4B) for size selection of cDNA,
and would share their opinion on performance/price? Or maybe you may
recommend some better (performance/price) alternative for getting rid of
adapters, dNTPs, short fragments after the second strand synthesis and
adapter ligation, i.e. DNA elution after gel-electrophoresis?

Many thanks in advance,
Lubomir S.

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