Drying agarose gels

D. KIM dkim at NMSU.Edu
Fri Mar 19 14:12:10 EST 1999


Well, this morning I found an agarose minigel dried onto its casting tray.
It was quite flat.  When I soaked it in water, it peeled off like a piece
of "fruit leather".

Daniel

Dr. Fernando Rodriguez-Pascual <frodrigu at mail.uni-mainz.de> wrote:
: I will be very grateful if someone can give me some tips to dry agarose
: gels. The purpose is to perform a kind of electrophoretic mobility shift
: assay to look the possible interaction between a 560 bp radioactive
: transcript and a purified protein. For this transcript size I should use
: agarose instead PAGE, but I was told that the drying of agarose gels is
: not easy.

: Thank you in advance
:  
: -- 
: #############################################
: Dr. Fernando Rodríguez Pascual
: Department of Pharmacology
: Johannes Gutenberg University
: Obere Zahlbacher Str. 67
: D-55101 Mainz, Germany
: Tf. +49 (6131) 17 68 36 or 17 32 45
: Fax. +49 (6131) 17 66 11
: email: frodrigu at mail.uni-mainz.de
: #############################################



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