I want to erase GPI anchor of GFRa-1, which is the last residue of mature
GFRa-1. At the C-terminal of GFRa-1 precursor protein, there are twenty or
more hydrophobic residues which will be cut and leave in the ER membrane.
The last residue of the new c-terminal will be the GPI anchor.
I want to get soluble GFRa-1 expressed in vivo, so I must erase the GPI
anchor. I plan to erase the c-terminal twenty or more hydrophobic residues
and GPI anchor use PCR. Is it enough to free the GFRa-1 from cell expressing it?
Beijing Medical University