IUBio

separation of small DNA fragments

Rich Dudley rdudley+ at pitt.edu
Fri Mar 19 12:51:10 EST 1999


Craig Neylon wrote:

> I am interested in finding a high resolution method for discriminating
> between approximately 100bp PCR products that differ by about 9 to 12
> bp.  I would like to know what the best agarose-based product would be.
> Right now my only option is FMC NuSieve 3:1 agarose. Thanks in advance.
>
> Dorian Henderson

I have had great success with Amresco's Agarose SFR.  We used a 4% gel to
distinguish two 17-mers from their 34bp annealed linker.  You can find them
at www.amresco-inc.com.

rich

--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
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