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CaPO transfection theories?

Wolfgang Schechinger Wolfgang.Schechinger at med.uni-tuebingen.de
Sat Mar 20 16:04:27 EST 1999


Demetrios, 

My personal non proven theory is this:
Calcium phosphate, something similar to "Ca3(PO4)2" is quite 
insoluble (you'll see the crystals under the microspcope). Since DNA 
contains phosphate, you'll get a kind of co-preciptation with mixed 
crystals (DNA,Ca)n(PO4)m, since calcium also complexes DNA 
molecules. 
A model could be that during crystal growth, 
DNA-phosphates coordinate with calcium ions already built in the 
crystal, but not yet saturated with phosphates. 
Crystals snow down on the cells and will be taken up by endocytosis 
and membrane invaginations/turnover or simply entering the cells by 
mechanical force since the are kind of sharply shaped.

Maybe somebody @ bionet can correct me, knows more or will even point 
to some Xray papers?

Wolfgang

> Date:          20 Mar 1999 20:35:39 GMT
> From:          dkalaitz at bu.edu (Demetrios Kalaitzidis)
> Reply-to:      dkalaitz at bu.edu (Demetrios Kalaitzidis)
> To:            "bionet.molbio.methds-reagnts mail newsgroup" <bionet-news at dl.ac.uk>
> Subject:       CaPO transfection theories?

> Hello, anyone have any theories on the mechanism of the Calcium
> Phosphate coprecipitation transfection method? Nobody seems to know
> how it works, only that it works ! thanks, dk
> 
> 
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Wolfgang Schechinger   
Pathobiochemistry Dept.      
University of Tuebingen, Germany
email: wgschech at med.uni-tuebingen.de * wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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