My personal non proven theory is this:
Calcium phosphate, something similar to "Ca3(PO4)2" is quite
insoluble (you'll see the crystals under the microspcope). Since DNA
contains phosphate, you'll get a kind of co-preciptation with mixed
crystals (DNA,Ca)n(PO4)m, since calcium also complexes DNA
A model could be that during crystal growth,
DNA-phosphates coordinate with calcium ions already built in the
crystal, but not yet saturated with phosphates.
Crystals snow down on the cells and will be taken up by endocytosis
and membrane invaginations/turnover or simply entering the cells by
mechanical force since the are kind of sharply shaped.
Maybe somebody @ bionet can correct me, knows more or will even point
to some Xray papers?
> Date: 20 Mar 1999 20:35:39 GMT
> From: dkalaitz at bu.edu (Demetrios Kalaitzidis)
> Reply-to: dkalaitz at bu.edu (Demetrios Kalaitzidis)
> To: "bionet.molbio.methds-reagnts mail newsgroup" <bionet-news at dl.ac.uk>
> Subject: CaPO transfection theories?
> Hello, anyone have any theories on the mechanism of the Calcium
> Phosphate coprecipitation transfection method? Nobody seems to know
> how it works, only that it works ! thanks, dk
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