PEG 8000 will do in TSS one-step transformation.

Chen Linan chenlinan at 263.NET
Sat Mar 20 23:31:07 EST 1999


Hi,

I am using TSS onestep preparation and trnsformation of competent cell. Must
I use PEG 3350? Can I use PEG 8000 because I cannot find PEG 3350? In this
case, when incubation at 37 degree for 30-60 min, I replace LB with 1xTSS
with 20mM glucose added? 

chen linan




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