RT-PCR problem!!

[tahar aboulkassim]

Hi everyone!
I did an RT-PCR and I had a band with the right size. I purified this band
from the gel and dissolve it in tris buffer. then I used 1µl of this RT-PCR
product and run another PCR (All the parameters were the same indeed the
primers).But I always obtain a smear.
I have no explanation for that and I will be so grateful if someone gives me
an idea.

thank you so much for help.

A. Taha
E- mail : ellape at sprint.ca