RT-PCR of GC rich regions
jla at gbf.de
Mon Mar 22 04:30:05 EST 1999
Try 1 to 10% DMSO in the PCR-buffer.
Additionally there where some additives described in one of the GIBCO
newsletters last year but don´t ask me which one it was.
amherrera at CIGB.EDU.CU schrieb:
> Hi all,
> I need to amplify GC rich regions (%GC = 80) by RT-PCR and I
> have run with troubles using the conventional procedures.
> It seem to me that someone once recomended the use of
> some aditives like formamide but I do not remeber well.
> Could anybody lend me a working protocol to solve this?
> Many thanks to all
EMail: JLA at GBF.DE
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