How to digest 5' overhang ?
kirberg at bii.ch
Mon Mar 22 16:20:01 EST 1999
In article <36F2ACD1.FF0120BC at leonardo.ls.huji.ac.il>,
yoramg2 at LEONARDO.LS.HUJI.AC.IL (Yoram Gerchman) wrote:
> Greetings netters.
> Dose anyone have a good method for digest 5' overhang ? I tried Mung
> Bean but it cut into my gene. Will it be better in lower temperature
> (11C) a-la T4 polymerize?
With mung bean, make a titration series (several 1/5s) of the enzyme
and incubate at RT.
This worked for me.
Good luck, jorg
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