references on setting up a PCR lab

"David Brayford ", B&B-BMB D.BRAYFORD at CABI.ORG
Mon Mar 22 12:51:40 EST 1999


To: methods at net.bio.net

From: Gerry Boucher <gboucher at mediom.qc.ca>

>Subject: Setting up a PCR Lab
>
>Date: Tue, 16 Mar 1999 00:33:35 -0400
>
>
>I need somme documentation ( articles, book titles or internet 
>address) on how to establish a PCR laboratory.
>subjects: sample preparation, preparation of reagents, preventing 
>contamination, location of the PCR machine etc.
>
>Thanks Gerry
>-----------------------------------------------

Gerry

Check out the following:

Good luck.
Dave Brayford
CABI Bioscience


Cone, W. R. & Fairfax, M. R. (1993). Protocol for ultraviolet irradiation of
surfaces to reduce PCR contamination. PCR Methods and Applications, 3
Supplement: S15-S17.

Dieffenbach, C. W. & Dveksler, G. S. (1993). Setting up a PCR Laboratory.
PCR Methods and Applications, 3 Supplement: S2-S7.

Dieffenbach, C. W., Dragon, E. A. & Dveksler, G. S. (1995). Setting up a PCR
Laboratory.  In PCR Primer.  A Laboratory Manual. pp. 7-16.  (Eds
Dieffenbach, C. W. & Dveksler, G. S.).  Cold Spring Harbor Laboratory Press,
New York.

Dragon, E. A. (1993). Handling reagents in the PCR Laboratory. PCR Methods
and Applications, 3 Supplement: S8-S9.

Dragon, E. A., Spadoro, J. P. & Madej, R. (1993). Quality control of
polymerase chain reaction. In Diagnostic Molecular Microbiology: principles
and applications. pp. 160-168. (Eds. Persing, D. H., Smith, T. F., Tenover,
F. C., & White, T. J.). American Society for Microbiology, Washington D.C..

Hartley, J. L. & Rashtchian, A. (1993). Dealing with contamination:
enzymatic control of carryover contamination in PCR. PCR Methods and
Applications, 3 Supplement: S10-S14.

Madej, R. & Scharf, S. (1990). Basic equipment and supplies. In PCR
Protocols: A Guide to Methods and Applications. pp. 455-459. (Eds. Innis, M.
A., Gelfand, D. H., Sninsky, J. J. & White, T. J.). Academic Press, London.

McCreedy, B. J. & Callaway, T. H. (1993). Laboratory design and work flow.
In Diagnostic Molecular Microbiology: principles and applications. pp.
149-159. (Eds. Persing, D. H., Smith, T. F., Tenover, F. C., & White, T.
J.). American Society for Microbiology, Washington D.C.

Orrego, C. (1990). Organizing a laboratory for PCR work. In PCR Protocols: A
Guide to Methods and Applications. pp. 447-454. (Eds. Innis, M. A., Gelfand,
D. H., Sninsky, J. J. & White, T. J.). Academic Press, London.




More information about the Methods mailing list