RT-PCR of GC rich regions
jla at gbf.de
Mon Mar 22 08:20:58 EST 1999
I forget to mention, for the RT you can use Superscript from GIBCO, works
up to 50° C or Tth works up to 75° C.
> amherrera at CIGB.EDU.CU schrieb:
> > Hi all,
> > I need to amplify GC rich regions (%GC = 80) by RT-PCR and I
> > have run with troubles using the conventional procedures.
> > It seem to me that someone once recomended the use of
> > some aditives like formamide but I do not remeber well.
> > Could anybody lend me a working protocol to solve this?
> > Many thanks to all
> > DiogenesQV
> J. Lauber
> Mucosal Immunity
> Mascheroderweg 1
> D-38124 Braunschweig
> Tel.: 0049-(0)531-6181-306
> Fax.: 0049-(0)531-6181-306
> EMail: JLA at GBF.DE
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