RT-PCR of GC rich regions

Jo Lauber jla at gbf.de
Mon Mar 22 08:20:58 EST 1999


I forget to mention, for the RT you can use Superscript from GIBCO, works
up to 50° C or Tth works up to 75° C.
Good luck

Jo


> amherrera at CIGB.EDU.CU schrieb:
>
> >     Hi all,
> >     I need to amplify GC rich regions (%GC = 80) by RT-PCR and I
> >     have run with troubles using the conventional procedures.
> >
> >     It seem to me that someone once recomended the use of
> >     some aditives like formamide but I do not remeber well.
> >
> >     Could anybody lend me a working protocol to solve this?
> >
> >     Many thanks to all
> >     DiogenesQV
>
> J. Lauber
> GBF
> Mucosal Immunity
> Mascheroderweg 1
> D-38124 Braunschweig
> Tel.: 0049-(0)531-6181-306
> Fax.: 0049-(0)531-6181-306
> EMail: JLA at GBF.DE




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