HOW TO GET A Sharp fragment using PCR product as template

Michael Schaefer schae at komma.zedat.fu-berlin.de
Tue Mar 23 06:50:47 EST 1999


Hi,

try to design nested or heminested primer sets. Most probably, the
second band will dissappear. The inner primer set may even overlap the
sequence of the outer primers by up to 70-80% of the primer length. A
gel-purification between 1st and 2nd PCR may be omitted in some - but
not all - cases. Good luck...

Michael Schaefer
FU Berlin, Germany
schae at zedat.fu-berlin.de



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