I have just begun using the Talon resin from clontech to purify my his
tagged protein. On the first run I used 200 mM L-histidine to elute
from the column. The column turned brown and then this colur eluted
form the column leaving the column clear not pink as is usual with
this column resin. Obviously the metal has leached from the column.
Is this normal when using histidine to elute from heavy metal affinity
columns? Is there a buffer system to use to avoid this?
R.Seymour at prospect.anprod.csiro.au