storing low concentrations of DNA
bmbthc at removebmb.leeds.ac.uk
Wed Mar 24 07:41:56 EST 1999
On 17 Mar 1999,
Andrew G. wrote:
> I am seeking advice on storing low concentrations of DNA fragments
> competitive PCR.
> What buffer + additives?
> What temperature for storage?
Andrew - When working with low concentration DNA solutions, you MUST
take into account the adhesion of your
DNA to polypropylene tubes. Surprisingly, the binding capacity of
different makes and types of polypropylene
tubes can be enormous! See 'Technical Tips Online' at the following
location for a paper on the subject:
http://www.biomednet.com/db/tto (article is under the 'Purification
P.S. You will need to join BioMednet to access the pages but it's free
and simple to do.
P.P.S. No affiliation to the authors, just thought this might be
School of Biochem. & Mol. Biol., University of Leeds, UK.
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