storing low concentrations of DNA

Hedley Carr bmbthc at removebmb.leeds.ac.uk
Wed Mar 24 07:41:56 EST 1999


On 17 Mar 1999,
 Andrew G. wrote:
 
 > Hi
 > I am seeking advice on storing low concentrations of DNA fragments
for
 > competitive PCR.
 > What buffer + additives?
 > What temperature for storage?
 >
 > thanks
 > Andrew
 

Andrew - When working with low concentration DNA solutions, you MUST
take into account the adhesion of your
DNA to polypropylene tubes. Surprisingly, the binding capacity of
different makes and types of polypropylene
tubes can be enormous! See 'Technical Tips Online' at the following
location for a paper on the subject:

http://www.biomednet.com/db/tto   (article is under the 'Purification
methods' title).

P.S. You will need to join BioMednet to access the pages but it's free
and simple to do.

P.P.S. No affiliation to the authors, just thought this might be
helpful!

Hedley Carr
School of Biochem. & Mol. Biol., University of Leeds, UK.



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