storing low concentrations of DNA

Hedley Carr bmbthc at removebmb.leeds.ac.uk
Wed Mar 24 07:41:56 EST 1999

On 17 Mar 1999,
 Andrew G. wrote:
 > Hi
 > I am seeking advice on storing low concentrations of DNA fragments
 > competitive PCR.
 > What buffer + additives?
 > What temperature for storage?
 > thanks
 > Andrew

Andrew - When working with low concentration DNA solutions, you MUST
take into account the adhesion of your
DNA to polypropylene tubes. Surprisingly, the binding capacity of
different makes and types of polypropylene
tubes can be enormous! See 'Technical Tips Online' at the following
location for a paper on the subject:

http://www.biomednet.com/db/tto   (article is under the 'Purification
methods' title).

P.S. You will need to join BioMednet to access the pages but it's free
and simple to do.

P.P.S. No affiliation to the authors, just thought this might be

Hedley Carr
School of Biochem. & Mol. Biol., University of Leeds, UK.

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net