Blotting RNA from Acrylamide to Nylon

Jonathan Howarth jrh2 at st-andrews.ac.uk
Wed Mar 24 12:36:53 EST 1999


Hello,

I'm wanting to blot RNA products from Ribonuclease protection assays from
5% acrylamide gels (native or denaturing possible) on to nylon membranes.
Can I do this by capillary blotting or by electroblotting using a
western-transfer apparatus (or similar). If anyone has any idea how this
is best done I would be grateful for your help.

Thanks,

Jonathan Howarth

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