IUBio

Need help w/ SDS & extraction buffer

Kelvin Luther umluther at cc.umanitoba.ca
Wed Mar 24 12:20:23 EST 1999


Kelly Luther wrote:
> 
> Mark Warner wrote:
> >
> > To all, I'm trying out a new RNA extraction buffer that contains Tris
> > (0.2M), sodium perchlorate (8M)(pH 8.3) and SDS (2%), but the SDS comes
> > out of solution and I can't seem to resolubilize it. I know this can
> > happen with other buffers, and usually mild heat will do the trick, but
> > it doessn't work. Any ideas?
> >
> > Mark
> 
> The larger the cation, the higher the temperature SDS will precipitate
> at.  If the sodium is precipitating the SDS, try lowering the
> temperature before it is mixed.  A smaller cation (Lithium) probably
> isn't possible is it?  If so you can try this as well.
> 

I couldn't understand why I was getting helpful SDS hints in my mail
this morning.  What I said above was correct with the exception of one
glaring error (is my face red!).  The larger the cation, the higher the
temperature SDS will come out of solution.  Cooling it will only make
the situation worse.  

-- 
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
*Kelvin Luther 
*University of Manitoba                                             
*Department of Chemistry    "Illegitimati non carborundum"    
*Winnipeg, Manitoba           General Joseph W. Stilwell      
*Canada                                                     
* mailto:umluther at cc.umanitoba.ca                           
*genealogy website: http://home.cc.umanitoba.ca/~umluther/  
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



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