IUBio

Southerns--need help!

sokching p12465 at PKPEL.CC.UKM.MY
Fri Mar 26 02:57:19 EST 1999


I'm having problems with my southern blots. The probe is hybridizing to
everything....therefore a smear appears on the autorad. As I'm using the
ECL kit, the hybridization temperature cannot exceed 42C. I've tried
increasing the stringency of the washing but it doesn't help. I'd
appreciate any ideas. Thanks.

sokching




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