What is the different usage between SDS and Sarkosyl?

Victor Levenson levenson at uic.edu
Thu Mar 25 20:09:47 EST 1999

"Wilson" <netson at hotmail.com> wrote:

>Dear all,
>Both SDS and sarkosyl are detergent, what is the different and different
>usage between them?
>Thanks a lot

For one thing SDS will form a precipitate with Cs (as in CsCl) or K,
while sarkosyl will not.

 Formation of SDS-K complexes was used for isolation of very stable
DNA-protein complexes many years ago. When I was involved in these
studies and had to characterize the DNA-protein ratio of the complexes
by their byouant (sp?) density I had to use Sarkosyl in CsCl gradients
since it does not form precipitates with Cs ions.

There may be other differences, though.....



More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net