In article <36E824FA.7AFE3B92 at ib3.gmu.edu>, Paul Rasmussen
<paul at ib3.gmu.edu> wrote:
>Gentra makes a DNA extraction kit for blood, tissue fluids etc which
>conains a cell lysis solution. Samples in this solution are stable for
>long periods of time at room temp (or even 37degrees.) I've used it
>frequently for blood and semen samples, though relatively easy protocols
>are available for tissue (mouse tails, biopsys etc...) The kit usually
>yields lots of good quality (OD260/280 ~1.9) DNA.
Really? That's the 'Generations' kit, yes?
We were looking at that kit and did some scans (220 nm -> 320 nm) and
found there was a lot of something in there that was not DNA. Some of the
samples came out vaguely brown, too, as if there was haem carry-through.
Oh, and the DNA comes out at pH 9 - 10, too.
Richard P. Grant MA DPhil | rgrant at cmtech.co.uk
work: www.cmtech.co.uk | home: www.avnet.co.uk/adastra
-- Doctorum Adamus cum Flabello Dulci --