Protein extraction from frozen tissues?

Samuel Gray samuel.gray at
Fri Mar 26 04:22:41 EST 1999

Does anyone have a good protocol for extracting total protein (for western
blotting) from tissues frozen in liquid nitrogen? Should I pulverise the
tissue whilst still frozen (as I would for RNA extraction) or allow the
tissues to defrost and mince/sonicate in a protein extraction buffer?

Thanks for any suggestions,

Samuel Gray.

Samuel Gray
Division of Vascular Medicine,
Derbyshire Royal Infirmary,
Tel: 01332 254966
Fax: 01332 254968
samuel.gray at

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