Storage of dry DNA? Any experience?
Richard P. Grant
r_grant at see.sig.for.address
Fri Mar 26 03:43:57 EST 1999
In article <36FAAED0.695F43CB at ib3.gmu.edu>, Paul Rasmussen
<paul at ib3.gmu.edu> wrote:
>No, This is the DNA puregene kit. I don't have any experience with a
D'oh! I should have realized when you were talking about pellets!
>the confusion lies in the methods of extraction and/or precipitation.
Yes, 'Generations' comes off the column in solution, but 'Puregene' is a
precipitation. Any gunk carry-through in Puregene (salting-out, isn't
it?) probably arises from red blood cell lysis.
>you for pointing out the shortcomings of this kit, as I would not want to
>lead anyone down the wrong path.
It's (Generations) fine for PCR of small loci, but I wouldn't want to use
it for much else. YMMV of course.
Richard P. Grant MA DPhil | rgrant at cmtech.co.uk
work: www.cmtech.co.uk | home: www.avnet.co.uk/adastra
-- Hiroshima '45 - Chernobyl '86 - Windows 98 --
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