Purification of palmitoylated proteins?
seanpat at FMED2.UNCU.EDU.AR
Fri Mar 26 15:56:16 EST 1999
>Is anyone aware of an affinity technique (or the like) that will
>selectively purify fatty acid modified proteins (specifically
>--- --- --- -- -- -- --- --- ---
>Richard J. Dudley (rdudley+ at pitt.edu)
>Research Specialist V
>Dept. of Cell Biology and Physiology
>University of Pittsburgh
>---> search BIONET archives at http://www.bio.net <---
I have used hydroxylamine treatment of extensively washed membranes
to purify a fraction substantially enriched in (de)palmitoylated proteins.
This gave me a fraction that could then be re-palmitoylated using membranes
and 3H-palmitate. If this serves your purpose, I'll pass on the protocol.
Sean Patterson, Ph.D.
Catedra de Fisiologia, CC33
Facultad de Ciencias Medicas
Universidad Nacional de Cuyo
Tel: (0261) 430-9385
Fax: (0261) 449-4117
e-mail: seanpat at fmed2.uncu.edu.ar
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