Temperature of long-term DNA storage

Nick Theodorakis theodorn at gusun.georgetown.edu
Fri Mar 26 13:24:52 EST 1999

In article <199903260134.MAA11313 at bohm.anu.edu.au>,
  s3148487 at STUDENT.ANU.EDU.AU (Brendan Scott) wrote:
> G'day!
> My lab is currently debating the pros & cons of what temperature is best
> for storing DNA over the long-term ie 10 years, etc..  It has been
> suggested that storing DNA at -20C is bad, because if it is in a salt
> solution e.g. TE, it will undergo a constant freeze-thaw cycle that will
> break up the DNA.  Manuals recommend 4C, but never say why.  While 4C has
> worked in the past, there is a tendency for a minority of DNA samples to
> grow fungi, bacteria and other colonies of lifeforms - which, contamination
> aside, will presumably be producing working nucleases as well.  While
> storing at -70C is an option, we have far more space at 4C and -20C.  What
> are other people's experiences re this dilemma?

I've been storing plasmid DNA in TE in the refrigerator, and noticed no loss
of quality after more than 4 years. But then I haven't had the problem of
microbial contamination, either.

| Nick Theodorakis                              |
| theodorn at gusun.georgetown.edu                 |

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