electroblot "swirlling"

Chad Rappleye rappleye at biomail.ucsd.edu
Sat Mar 27 02:10:43 EST 1999


I am transferring proteins from SDS-PAGE to nitrocellulose by
electroblotting.  When I stain the blot following transfer, the proteins
have transferred but the lanes are all "swirlled" around in a radial
pattern!?!  Obviously, this makes the blot useless - anyone know why or
have a solution?

--Chad



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