IUBio

protein expression in PLysS

Anant Patkar apatkar at biosci.cbs.umn.edu
Sat Mar 27 20:14:17 EST 1999


We have also observed leaky expression in E. coli BL21(DE3) with
pET23-based 
vector.  There was also significant plasmid stability problem.  Because
of the leaky expression, only 60% of the cells would have plasmid after
reaching stationary phase.  Has anyone seen this?  

Anant Patkar
University of Minnesota



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