IUBio

extract RNA from paraffin section

alvin fungalvin at hotmail.com
Sun Mar 28 04:02:19 EST 1999


HI ya,
No matter RNA or DNA, first of all you need to remove the paraffin.
Use xylene to remove the paraffin, then rehydrate with absolute ethanol.
Repeat twice or more until all paraffin is gone. Then, you can follow the
RNA extraction steps you experienced.
Michael Wing-Yan CHAN <bomchan at ust.hk> wrote in message
news:Pine.GSO.3.95L.990325162240.9023A-100000 at uststf3...
> Dear all,
>
>     I want to extract RNA from paraffin section and use it
> to perform RT-PCR, do you have any suggestions of how to
> extract it.
>
>    Thanks
>
> yours
> Michael
> ------------------------------------------------------------------------
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