6x his tag protein purification

Yoram Gerchman yoramg2 at LEONARDO.LS.HUJI.AC.IL
Mon Mar 29 13:31:32 EST 1999


We use 300mM Imidazol (pH tit rated to 7.9). Low pH should work but it
lethal for some proteins.
.........................................................................
"Support bacteria - they're the only culture some people
have"                      
               (anonymous)
Yoram Gerchman
Division of Microbial & Molecular Ecology
The Institute of Life Sciences
Hebrew University of Jerusalem
Givat Ram, Jerusalem 91904,
Israel



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