remove specific RNA with beads?

Nicole Focks focks at zedat.fu-berlin.de
Mon Mar 29 11:09:35 EST 1999


Hi,

has anybody ever tried to remove unwanted RNA from total RNA by magnetic beads?
I tried to bind unwanted RNA by a specific biotin-labelled oligo coupled
to the beads by streptavidin but still have lots of this RNA species in my
samples. Does anybody have an idea which conditions to take or if this
method is usefull for quantitative applications at all?

Thanx,

Nicole



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