KI and DNA purification

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.ucsf.edu
Mon Mar 29 22:52:08 EST 1999


In article <7dp5pu$7ht$1 at nnrp1.dejanews.com>, jag100 at snethen.com wrote:

> I have inherited a protocol for purifying DNA from agarose gels with
> Promega's AgarACE enzyme.  The protocol I have calls for melting the gel
> slices in 5.2M KI (final conc ~1.7M in ~300 ul total volume) before adding
> the AgarACE.

[snip]

> Thanks,
> Jennifer

Is that really KI?  I thought most protocols user periodate
(KIO4) or perchlorate for dissolution of agarose.  Sorry to
question you, its just that the idea of using KI is new to me.
     Bernard
-- 
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA



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