KI and DNA purification

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.ucsf.edu
Mon Mar 29 22:52:08 EST 1999

In article <7dp5pu$7ht$1 at nnrp1.dejanews.com>, jag100 at snethen.com wrote:

> I have inherited a protocol for purifying DNA from agarose gels with
> Promega's AgarACE enzyme.  The protocol I have calls for melting the gel
> slices in 5.2M KI (final conc ~1.7M in ~300 ul total volume) before adding
> the AgarACE.


> Thanks,
> Jennifer

Is that really KI?  I thought most protocols user periodate
(KIO4) or perchlorate for dissolution of agarose.  Sorry to
question you, its just that the idea of using KI is new to me.
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA

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