In article <bpmurray*STUFFER*-2903992052080001 at macmac-2.ucsf.edu>,
bpmurray*STUFFERfirstname.lastname@example.org (Bernard P. Murray, PhD) wrote:
> In article <7dp5pu$7ht$1 at nnrp1.dejanews.com>, jag100 at snethen.com wrote:
>> > I have inherited a protocol for purifying DNA from agarose gels with
> > Promega's AgarACE enzyme. The protocol I have calls for melting the gel
> > slices in 5.2M KI (final conc ~1.7M in ~300 ul total volume) before adding
> > the AgarACE.
>> > Thanks,
> > Jennifer
>> Is that really KI? I thought most protocols user periodate
> (KIO4) or perchlorate for dissolution of agarose. Sorry to
> question you, its just that the idea of using KI is new to me.
> Bernard P. Murray, PhD
> Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA
Yep, it's KI that I use (I've made it myself several times)--although since I
inherited the protocol, it's very possible that there was a typo
way-back-when.... That's part of what I'm trying to figure out.
I believe GeneClean (Bio101?) uses NaI, not KI, in their kit.
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